ktmal  (StatLab Medical Products Inc)

Journal: International Journal of Molecular Sciences

Article Title: Cardiac Functional and Structural Abnormalities in a Mouse Model of CDKL5 Deficiency Disorder

doi: 10.3390/ijms24065552

Figure Lengend Snippet: Myocardial fibrosis in the heart of Cdkl5 +/− mice. ( A ) Representative images of hematoxylin and eosin staining of longitudinal sections of the ventricular tissue of Cdkl5 +/+ (left panel) and Cdkl5 +/− (right panel) mice. Scale bar = 50 μm. ( B ) Representative images of Masson’s trichrome staining of longitudinal ( a , b ) and transverse ( c , d ) sections of the ventricular tissue of Cdkl5 +/+ (left panels) and Cdkl5 +/− (right panels) mice showing an increase in blue-stained collagen fibers in the heart of Cdkl5 +/− mice compared to wild-type (+/+) mice. Scale bars = 50 μm. ( C ) Expression of collagen type I alpha 2 chain ( Col1a2 ) and collagen type III alpha 1 chain ( Col3a1 ) in ventricular tissue isolated from the heart of 3-month-old Cdkl5 +/+ (n = 7) and Cdkl5 +/− (n = 8) mice. Data are presented as fold change in comparison with cardiac tissue from Cdkl5 +/+ mice. ( D ) Histogram showing quantification of vimentin protein levels normalized to GAPDH in protein extracts of ventricular tissue from 3-month-old Cdkl5 +/+ (n = 5) and Cdkl5 +/− (n = 5) mice. Data are expressed as percentages of Cdkl5 +/+ mice. Example of immunoblots of three animals from each experimental group on the right. Values in ( C , D ) are presented as means ± SEM; * p < 0.05 (two-tailed Student’s t -test).

Article Snippet: Sections were stained with Masson’s trichrome (MT) using Masson’s Trichrome Stain Kit-Mallory’s (StatLab, KTMAL); the procedure was carried out according to the protocol included with the kit.

Techniques: Staining, Expressing, Isolation, Comparison, Western Blot, Two Tailed Test

Journal: Journal of Tissue Engineering

Article Title: Tenogenically differentiated adipose-derived stem cells are effective in Achilles tendon repair in vivo

doi: 10.1177/2041731418811183

Figure Lengend Snippet: Achilles tendon sections (n = 4 per group and per time point) stained with Mallory’s Trichrome: representative images of tendon sections showing matrix organization at (a) 1.5, (b) 3, and (c) 4.5 weeks post excision injury: (1a–c) Gp1 (Defect Only), (2A–C) Gp2 (Defect + Gel), (3a–c) Gp3 (Defect + Gel + Undifferentiated ADSCs), (4a–c) Gp4 (Defect + Gel + Tenogenically Differentiated ADSCs), (Nta–c) normal tendon; 100x, Scale bar = 0.2 mm; the mean histological grading scores (d) and the mean collagen fiber organization scores (e) of tendon sections; normal tendon (Nt); * p < 0.05 and ** p < 0.01 denote significance with time.

Article Snippet: Upon mounting onto slides and rehydration, slides were stained with picrosirius red and Mallory’s Trichrome (MT) staining kits (American MasterTech).

Techniques: Staining